Antibiotic susceptibility of staphylococcus aureus isolated from nasal hisopathies in students of the Faculty of Health Sciences, Universidad Peruana Los Andes - Huancayo

Abstract

The general objective of the research was to determine the antibiotic susceptibility of Staphylococcus aureus isolated from nasal swabs in students of the Faculty of Health Sciences of the Universidad Peruana Los Andes. The observational method was used with a transverse non-experimental design, being a study of basic type and descriptive level. The population consisted of all S. aureus cultures isolated from students, working with 78 isolated S. aureus cultures and identified from nasal samples of 345 students selected by stratified sampling by proportional affixing, which were seeded in Mannitol salty agar and blood Azide (incubated for 24 hours at 37°C). Identification was performed by Gram staining and coagulase, catalase and DNAsa tests. The determination of antibiotic susceptibility was performed using the diffusion disc method (Kirby-Bauer technique) with twelve antibiotics according to the methodology and cut-off points proposed by the National Institute of Health (INS). S. aureus was isolated and identified with a frequency of 22,6%; The majority of carriers were between 22 and 24 years old (56,4%), with a greater distribution in the female sex (53,8%). Antibiotic susceptibility showed that 48,7% of the isolates were sensitive to gentamicin and trimethoprim/sulfamethoxazole; 39,7% intermediate to vancomycin and 32,1% resistant to rifampicin.